New publication in Nucleic Acids Research
In this work, we could show that RNA binding of cold shock domains (CSDs) goes way beyond the typical single domain-RNA contacts. The non-canonical CSDs are involved in direct RNA contacts and at least three domains (CSD789) synergistically bind RNA. Also, we present for the first time a complex structure between poly(A)-binding protein and Upstream-of-N-Ras. Both proteins have been shown to interact during translation initiation. Thus, this work is a major contribution towards understanding regulation of translation initiation.
Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein